1. Nikon Eclipse Me600 Manual Transmission
2. Nikon Eclipse Me600 Manual Download
3. Nikon Eclipse Me600 Manual User

All Manual All Motorized Leica Leitz / Reichert Meiji Mitutoyo Nikon Eclipse 80/90i Eclipse ME600/400 IC66 L150 L200 LV100/150 MM 40/60/200/400 MM 800 Optiphot 100/M Optiphot 150 Optiphot 200 Optiphot 300 SMZ TE2000/Ti-U TE300 Olympus Zeiss Punch/Probe Needles. Microscope w/ camera (manual). Nikon Eclipse ME600. Location: Cleanroom. Primary Training Contact: Kirk Bryson. Operating Procedures: Microscope Camera Operation.pdf. Thank you for purchasing the Nikon product. This manual is written for the users of the Nikon Simple Polarizing Attachment for ECLIPSE i series. To ensure correct usage, read this manual together with the instruction manual supplied with the microscope.

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An optical microscope equipped with five objectives and Deltapix Invenio digital camera. The microscope can be used in differential interference contrast (DIC) imaging, which is an imaging method based on the contrast difference of the samples. With DIC it is possible to see details from optically transparent samples which are invisible in the ordinary microscope images.

• Optics: 5X/0.15, 10X/0.30, 20X/0.46, 50X/0.80, 100X/0.90
• Option for differential interference contrast (DIC) imaging

Last updated: 8.1.2013

Nikon Eclipse Me600 Manual Transmission

Description:

Nikon Eclipse ME600 is optical microscope equipped with three objectives (20x, 50x and 100x) and an incident (epi) illumination system. The microscope can be used in differential interference contrast (DIC) imaging, which is an imaging method based on the contrast difference of the samples, and in dark field (DF) mode. With DIC and DF, it is possible to see details from optically transparent samples that are invisible in the ordinary microscope images.

Working principle

Differential Interference Contrast (DIC) This method, an extension of polarization contrast, is suitable for the visualization of even minute differences in height on surfaces. A birefringent prism (4) is used which splits the polarized light beam into two partial beams on its way to the sample. These two partial beams strike the sample (6) with lateral displacement from each other. If the surface is completely flat, nothing will happen. However, if there is a small step between the partial rays, one of the two beams has to travel along a path which is 2Δh longer. Once the partial beams have returned via DIC prism (4) and analyzer (7), they display the same direction of vibration again and can interfere with each other in the intermediate image. The path difference experienced on the surface then changes into grey values which can be seen by the eye: steps become visible in the form of relief. As an auxiliary object, the lambda-plate (7a) finally changes the grey values into colors again. Dark Field (DF)

Nikon Eclipse Me600 Manual Download

The light is emanating from the illumination lamp and collimated by the Collector. Most of the light is then blocked by the Condenser-aperture-diaphragm, which has a central stop that blocks the central rays of the beam. The light is then reflected by a 45° mirror with a circular hole in the center. In this way the light travels towards the sample on the outside of the actual objective lens. It is then focused on the sample by an inner mirror at the end of the objective housing. Light is deflected by any unevenness on the sample surface, and part of it is passed back via the objective lens to the eye of the operator. In this way, no directly (specularly) reflected light is seen by the operator (i.e. a perfectly clean mirror surface appears black). Only height differences, given by the dust and edges, on the surface are seen in the image.

Nikon Eclipse Me600 Manual User

Bright field (left) and dark field (right) images of a test sample. In DF only the edges are visible.